ALTERATIONS IN EXTRACELLULAR MATRIX AND CONNEXIN 43 IN THE OFFSPRING HEART FROM DIABETIC RATS

HUGO SATO; DANIEL MUSIKANT; SABRINA LORENA ROBERTI; ALICIA JAWERBAUM; ROMINA HIGA

Mar del Plata

Congreso; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2018

Maternal diabetes leads to the programming of metabolic and cardiovascular alterations in the adult offspring. The pathogenesis ofdiabetes-induced heart alterations involves remodeling of extracellular matrix (ECM) components and deposition of collagen. In thiscontext, the role of matrix metalloproteinase 2 (MMP2) is relevant.MMP2 overactivation is involved in pathological processes. BesidesECM components, MMP2 is able to cleave intracellular molecules as&RQQH[LQPDLQFRPSRQHQWRIWKHJDSMXQFWLRQVDQGKHPLFKDQnels in myocytes, affecting the physiological function of the chanQHOV2XUREMHFWLYHZDVWRHYDOXDWHZKHWKHUWKHUHDUHDOWHUDWLRQVLQthe collagen deposition, MMP2 activity and expression of connexinLQWKHKHDUWRIWKHRIIVSULQJIURPGLDEHWLFUDWV0HWKRGRORJ\3UHJHVWDWLRQDO GLDEHWLF UDWVZHUH REWDLQHG E\ QHRQDWDO VWUHSWR]RWRFLQDGPLQLVWUDWLRQPJNJDQGDWPRQWKRIDJHZHUHPDWHGZLWKhealthy males. Heart of male adult offspring of 5 month of age fromFRQWURO DQG GLDEHWLF UDWV ZHUH H[SODQWHG DQG OHIW YHQWULFOHV ZHUHstore for histological determinations, qRT-PCR and Western blot.5HVXOWVP51$OHYHOVRI&ROODJHQ,DQG,,,ZHUHLQFUHDVHGDQGIROGUHVSHFWLYHO\SLQFRQFRUGDQFHZLWKDQLQFUHDVHGLQWHUVWLWLDOFROODJHQGHSRVLWLRQREVHUYHGLQ0DVVRQ·V7ULFKURPHVWDLQing in the left ventricles of the offspring from diabetic rats comparedWRFRQWUROV$FWLYLW\RIERWKSUR003DQG003ZDVLQFUHDVHGLQWKHOHIWYHQWULFOHVRIWKHRIIVSULQJ?VKHDUWIURPGLDEHWLF UDWV DQGWLPHVUHVSHFWLYHO\S7KHH[SUHVVLRQRIFRQQH[LQZDVIRXQGUHGXFHGLQWKHRIIVSULQJ?VOHIWYHQWULFOHIURPGLDEHWLFrats compared to controls (25%, p