ALTERATIONS IN THE MTOR PATHWAY IN THE DECIDUA OF DIABETIC RATS DURING EARLY POST-IMPLANTATION

SABRINA LORENA ROBERTI; EVANGELINA CAPOBIANCO; HUGO SATO; ROMINA HIGA; ALICIA JAWERBAUM

Mar del Plata

Congreso; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2018

Maternal diabetes increases the risk of anomalies in the embryonic development. During early embryonic development the deciduaplays an important role in the histotrophic nutrition. In turn, the mTORSDWKZD\PHFKDQLVWLFWDUJHWRIUDSDP\FLQLVDQLQWUDFHOOXODUVLJQDOLQJSDWKZD\LQYROYHGLQQXWULHQWWUDQVSRUWDQGHPEU\RQLFJURZWKDQGdevelopment. LAT1 (L-amino acid transporter-1) and SNAT2 (Sodium-dependent neutral amino acid transporter-2) are transporters ofDPLQR DFLGV UHJXODWHG E\P725 SDWKZD\2EMHFWLYHWR VWXG\WKHWRWDODQGSKRVSKRU\ODWHGSURWHLQOHYHOVGRZQVWUHDPP725SDWKZD\(%3 H,)(ELQGLQJSURWHLQ$.7 \ US6 ULERVRPDOSURWHLQS6)) and the mRNA levels of mTOR, LAT1 and SNAT2, in the decidua from control and diabetic rats at day 9 of gestation. Methods:Diabetic Wistar rats, obtained by intraperitoneal administration ofVWUHSWR]RWRFLQPJNJZHUHPDWHGZLWKKHDOWK\PDOHV2QGD\RIJHVWDWLRQWKHGHFLGXDZDVREWDLQHGDQGSURFHVVHGWREHXVHGIRUPHDVXUHPHQWRIWRWDODQGSKRVSKRU\ODWHG(%3$.7DQGUS6E