Impact of two breast-milk derived potential probiotics on the gut microbiota of an obese child

ODDI, SOFIA; HUBER, PAULA; REINHEIMER, J.; BURNS, PATRICIA; VINDEROLA, GABRIEL; SIVIERI, KATIA

Miami

Simposio; 2019 Gut Microbiota for Health World; 2019

American Gastroenterological Association

This study was designed to evaluate the effects of two potential probiotic strains isolated from human breast milk, Lactobacillus plantarum 73A and Bifidobacteria animalis subsp. lactis INL1 on the microbiota of an obese child using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®), a dynamic model of the human gut.Methods:The SHIME was run on the following program: stabilization (2 weeks), L. plantarum 73A (2 weeks, 1010 CFU/ day), washout (1 week) and L. plantarum 73A/B. lactis INL1 (1010 CFU/ day each, for 2 weeks). Analysis of the intestinal microbial composition (16S rRNA gene amplicon sequencing) was performed, short-chain fatty acids (SCFAs) levels were measured by gas chromatograph and ammonium (NH4+) concentration was assessed by an ammonia selective ion electrode.Results:Both treatments significantly decreased ammonia concentration (or production?): from 483 ppm (stabilization) to 434 ppm (L. plantarum 73A, p = 0.000), and from 530 ppm (washout) to 460 ppm (mix of strains, p = 0.015). No changes in SCFA were observed. Both study strains survived in the SHIME and significantly modified microbiota structure (p= 0.04). Some Clostridium species decreased whereas Roseburia intestinalis significantly increased, a species linked to a healthy gut.Conclusion:Breast milk-derived L. plantarum 73A and B. animalis subsp. lactis INL1 were demonstrated to have potential for further assessed in gut health promotion in obese children.