Occurrence of lactobacilli with probiotic potential in Argentinian breast milk.

ODDI, SOFIA; REINHEIMER, JORGE A.; SOSA, IRINA; CONTINI, V.; BURNS, P.; BINETTI, ANA; VINDEROLA, GABRIEL

Singapur

Simposio; https://isappscience.org/2017-annual-meeting/; 2018

International Symposium of the International Scientific Association of Probiotics and Prebiotics

Background and RationaleDifferences in environmental and gut microbiota are factors that favor the exploration of locally sourcedprobiotics. Such probiotics target the particular populations to improve their gut health. This could lead to localdevelopments and activities which may pave the way to their application in social programs. Such probioticsare particularly beneficial for people in developing countries, who generally lack access to affordablecommercial probiotics and are more often exposed enteric infections.Objectives: Indicates the purpose of the studyTo explore the occurrence in breast milk samples collected in the city of Santa Fe (Argentina) of lactobacilliwith functional and technological potential to become locally sourced new probiotics.Methodology: Describe pertinent experimental procedures104 mothers donated 161 breast milk samples. Lactobacilli were isolated using MRS agar, preliminaryidentified by MALDI-TOF and then the identity was confirmed by partial 16S RNA sequencing. Hydrophobicitywas determined (hexadecane and xylene partition). Strains were freeze-dried in 10% lactose or 10%polidextrose and survival was assessed after accelerated (37°C, 4 weeks) or regular (5° and 25°C, 12 months)storage. Strains were co-cultured with murine RAW 264.7 macrophages for screening the capacity to inducethe anti-inflammatory cytokine IL-10.Results: Summarize the results of the research14 LAB isolates were obtained and identified as L. plantarum (7 isolates), L. gasseri (3), L. fermentum (2), L.rhamnosus (1) and L. gallinarum (1). Hydrophobicity ranged from 7.4 and 95.9%. L. gasseri 70a, 70b and 73awere the strains with higher hydrophobicity and capacity to induce IL-10 production by macrophages. Very lowcorrelation between survival at 37°C/1 month and 5°/12 months was observed. Better correlation at 25°C wasobserved for lactose (R2 = 0.77) compared to polydextrose (R2 = 0.65). Less than 1 log order of cell death wasobserved in polydextrose for L. gasseri 70a, 70b and 73a after 12 months of storage at 5°C.Conclusions: State the main conclusionsThree L. gasseri strains were isolated, which displayed promising functional and technological properties forfurther studies on their health promoting properties, to be used as locally sourced probiotic strains.