EFFECT OF LOW-DOSE GLYPHOSATE ON EMBRYO IMPLANTATION

ODDI, SOFIA; ALTAMIRANO GABRIELA ANAHI; GOMEZ AYELEN LUCIANA; ABUD JULIAN; MÓNICA MUÑOZ-DE-TORO; LAURA KASS

Buenos Aires

Congreso; XXIV ANNUAL MEETING OF THE ARGENTINEAN SOCIETY OF BIOLOGY (SAB); 2022

Sociedad Argentina de Biología

Although glyphosate-based herbicides are considered safe due to their low persistence, new evidence suggests that they could affect thecorrect embryo implantation and development even in low doses. The trophectoderm surrounding the blastocyst plays a pivotal role inthe invasion, migration, and spiral arteries remodeling from the decidua. This process is high-regulated, and its alterations could carryout preeclampsia, miscarriages, and other associated pathologies. Previous studies demonstrated that concentrations of 0.2 and 2 μM ofglyphosate (G) stimulated migration activity in a human endometrial carcinoma cell line (Ishikawa). This study aims to analyze ex vivothe effect of 2.5 μM G in murine blastocyst development. The cellular migration was also assayed using the trophoblast cell line HTR-8/SVneo with 0.625, 1.25, 2.5, 5, and 10 μM of G. E3.5 embryos, recovered from pregnant BALC/b mice, were placed on murine uterineepithelial cells monolayer with 2.5 μM of G or vehicle (V). The implantation area and hatching/attachment time were registered for sixconsecutive days. The wound healing assay was performed to evaluate the migration activity. The monolayer was pretreated with Gconcentrations for 24 h, and the medium was renewed after scratching. Then, the uncovered areas were registered at 0 and 12 h. Cellviability was determined spectrophotometrically after 24 and 48 h of treatment using WST-1 reagent and by counting cells in ahemocytometer. All the assays were performed in triplicate. The blastocyst implantation area (G: 0.47 ± 0.03 mm2; V: 0.32 ± 0.14 mm2)and hatching/attachment time (G: 42.3 ± 10.5 h; V: 45.2 ± 19.9 h) were similar between groups. Cellular migration was stimulated at0.625 μM G compared to V (P  0.05). These results suggest that even low concentrations of G could dysregulate some processesassociated with implantation.