GLYPHOSATE CONCENTRATION DETECTED IN HUMAN BREAST MILK ALTERS MILK PROTEINS LEVEL IN A MOUSE MAMMARY CELL LINE

IARA LISET ROTH; SOFÍA ODDI; AYELÉN LUCIANA GOMEZ; GONZALO SCHIERANO-MAROTTI; LAURA KASS; GABRIELA ANAHÍ ALTAMIRANO

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

Sociedad de Biociencias

Previously, we demonstrated that milk protein synthesis is affectedby environmental estrogens using a 3D in vitro model. In recentyears, the broad-spectrum herbicide glyphosate (GLY) has beenclassified as a potential estrogenic and androgenic compound.Here, our aim was to analyze whether direct exposure to glyphosate-based herbicide (GBH) and its active ingredient GLY affectsmilk proteins synthesis during mammary differentiation using themurine cell line HC11. Cells were grown in medium supplementedwith epidermal growth factor, insulin and fetal bovine serum (FBS)until confluent. Cells were then cultured for 72 h with medium supplementedwith 2% charcoal-stripped FBS, after which they weredifferentiated with lactogenic hormones (prolactin, insulin and dexamethasone)and exposed for 72 h to: a) Vehicle (phenol red-freemedium), b) 0.01 and 0.5 μM GBH and c) 0.01 and 0.5 μM GLY.The concentrations of GBH and GLY were selected considering thedetected values of GLY both in human milk and in serum and urineof pregnant women. After 72 h of differentiation and exposure toGBH and GLY, cell viability assay was carried out using the WST-1kit. Also, the mRNA levels of milk proteins: beta-casein (CSN2) andwhey acidic protein (WAP), considered markers of functional differentiation,were analyzed by real-time RT-PCR. No significant differencesin cell viability were found between the treatments. On theother hand, the mRNA level of CSN2 was increased in HC11 cellstreated with 0.01 μM GBH and GLY (p