SEARCHING FOR POTENTIAL TARGETS OF BENZOPHENONE- 3 ACTION INVOLVED IN THE MAMMARY GLAND ALVEOLAR GROW AND FUNCTION

GONZALO SCHIERANO-MAROTTI; GABRIELA ANAHÍ ALTAMIRANO; AYELEN LUCIANA GOMEZ; SOFÍA ODDI; MÓNICA MUÑOZ-DE-TORO; LAURA KASS

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

Sociedad de Biociencias

The UV-filter benzophenone-3 (BP3), commonly used in sunscreens,has been shown to alter the mammary gland development.Previously, we have shown that intrauterine exposure to BP3 altersthe histoarquitecture of the lactating murine mammary gland bymodifying the alveolar area, alveolar size ratio and the myoepitheliallinear density, without changing the expression of milk proteins. Potentialtargets of BP3 action involved in alveolar growth and functioncould be signal transducer and activator of transcription 5 (STAT5a),Ets transcription factor 5 (ELF5) and glucocorticoid receptor (GR).Therefore, our aim was to evaluate the expression of these moleculesin the lactating mammary gland of the F1 female offspring afterthe intrauterine exposure to BP3. Pregnant F0 C57BL/6 mice weredermally exposed to vehicle (sesame oil; control) or 50mg BP3/kg/day (50BP3) from gestation day 8.5 to 18.5. At 8 weeks old, F1 femaleoffspring were bred, and mammary gland samples were obtainedon lactation day 10. The mRNA expression of STAT5a, ELF5and GR was evaluated by real-time RT-PCR. The 50BP3 groupshowed similar expression of STAT5a, ELF5 and GR to the controlgroup (p > 0.05). In conclusion, STAT5a, ELF5 and GR do not seemto be involved in the alveolar modifications induced by intrauterineexposure to BP3 in the lactating murine mammary gland.