EFFECT OF LOW-DOSE PHOSATE ON EMBRYO IMPLANTATION

ODDI, SOFIA; ALTAMIRANO, GA; GOMEZ AL; ABUD J; MUÑOZ-DE-TORO M; KASS, L

Buenos Aires

Jornada; Jornada Anual SAB 2022; 2022

Sociedad Argentina de Biología

Although glyphosate-based herbicides are considered safe due to their low persistence, new evidence suggests that they could affect the correct embryo implantation and development even in low doses. The trophectoderm surrounding blastocyst plays a pivotal role in the invasion, migration, and spiral arteries remodeling from the decidua. This process is high-regulated, and its alterations could carry out preeclampsia, miscarriages, and others associated pathologies. Previous studies demonstrated that concentrations of 0.2 and 2 µM of glyphosate (G) stimulated migration activity in a human endometrial carcinoma cell line (Ishikawa). This study aims to analyze ex vivo the effect of 2.5 µM G in murine blastocyst development. The cellular migration was also assayed using the trophoblast cell line HTR-8/SVneo with 0.625, 1.25, 2.5, 5 and 10 µM of G. E3.5 embryos recovered from pregnant BALC/b mice were placed on murine uterine epithelial cells monolayer with 2.5 µM of G or vehicle (V). The implantation area and hatching/attachment time were registered for six consecutive days. The wound healing assay was performed to evaluate the migration activity. The monolayer was pretreated with G concentrations for 24h, and the medium was renewed after scratching. Then, the uncovered areas were registered at 0 and 12h. Cell viability was determined spectrophotometrically after 24h and 48h of treatment using WST-1 reagent and by counting cells in a hemocytometer. All the assays were performed by triplicate. The blastocyst implantation area (G: 0.47 ± 0.03 mm2; V: 0.32 ± 0.14 mm2) and hatching/attachment time (G: 42.3 ± 10.5 h; V: 45.2 ± 19.9 h) were similar between groups. Cellular migration was stimulated at 0.625 µM G compared to V (p  0.05). These results suggest that even low concentrations of G could dysregulate some processes associated with implantation.