HEPATOCYTE TRANSPLANTATION ISOLATED FROM RAT LIVERS WITH PRENEOPLASTIC FOCI. IMPACT OF AN IMMUNOSUPPRESSANT AGENT ADMINISTRATED TO THE RECIPIENTS

VERA MARINA C.; BIANCARDI,MARÍA E.; PISANI,GERARDO B.; BOTTAI, HEBBE; TABORDA, D.; LUGANO M.; QUINTANA,ALEJANDRA

Rosario

Congreso; XVI CONGRESS y XXXIV ANNUAL MEETING Sociedad de Biologia de Rosario; 2014

Sociedad de Biología de Rosario

Transplant of hepatocytes isolated from donor (D) with hepatic preneoplastic foci (PF) developed PF in the recipient (R) after 7 days postransplant. We compare the number of PF between R not immunosuppresed (NISR) and R immunosuppressed with Cyclosporin A (CsA) (ISR), and we analyze hepatic functionality in both group. 90-day Wistar male rats were used as D and as R. D were subjected to a 2-phase model of rat hepatocarcinogenesis to induce PF. Experimental groups: ISR, R recieved 20 mg/kg CsA 4 days before and 1 day after transplant; NISR were not immunosuppressed; Sham (controls). R was partial hepatectomized after transplant. At days 7, 21 and 60 R were anesthetized and bled to measure ALP, GOT and GPT. Pieces of liver were used for an immunohistochemisty using rGSTPi to identify PF. For each R the total area of tissue obtained was calculated with image software and PF were counted (nº PF/cm2 of tissue). 60-day R were used to calculate the nº of PF. ALP was statistically higher for NISR and for ISR than SHAM at 7 and 21 days; TGP was higher for NISR only at 7 days, GOT did not present differences between groups or time. The number of PF was significant higher for ISR at 60 days. We concluded that the number of foci increased after 60 days in ISR recipients and that hepatic function was conserved