HEPATOCYTE TRANSPLANTATION ISOLATED FROM RAT LIVERS WITH PRENEOPLASTIC FOCI. IMPACT OF THE AMOUNT OF CELLS TRANSPLANTED

VERA MARINA C.; BIANCARDI,MARÍA E.; PISANI,GERARDO B.; PISKULIC L.; CARRILLO M,; LUGANO M.; QUINTANA,ALEJANDRA

Zavalla

Congreso; XV CONGRESS y XXXIII ANNUAL MEETING Sociedad de Biología de Rosario; 2013

Sociedad de Biología de Rosario

Hepatocyte transplant is proposed as an alternative for patients waiting for hepatic transplant. The hepatocellular carcinoma is developed in 3 states: initiation (I), promotion (P) and progression. The subclinic IP state of the donor (D) liver could be dangerous for the recipient (R). We analyzed if R developed preneoplasia after being transplanted with hepatocytes isolated from livers with IP foci (IPF); and we correlated the number of transplanted cells with the presence of IPF in the R. Adult male Wistar rats (300-350 g) were used as D (n=6) and R. To induce IPF, Ds were subjected to a 2-phase model of rat hepatocarcinogenesis. Rs were partially hepatectomized and were divided in 2 groups (G): rats that received 30.000 (G30) or 150.000 (G150) hepatocytes. 3 D and 6 R per D were used for each G. Times of sacrifice: 7 and 21 days postrasplant (n=3). Samples of the whole liver were fixed in formaldehyde 10 % P/V and processed for paraffin embedded. The presence of IPF was determinate using anti rGST-P. The total area occupied by hepatic tissue (cm2) was calculated for each R by computerized image analysis and the number of IPF and IP isolated cells rGST-P positives (IPC) were counted. Results, expressed as means ± sd per unit of tissue area, were analyzed with ANOVA. No statistical differences were obtained for the amount of IPF or IPC, neither taking in account the sacrifice time not with the amount of cells transplanted. R developed IPF when they were transplanted with hepatocytes isolated from IP livers and the amount of cells used did not modify the number of IPF