PROBIOTIC LACTOBACILLUS REINFORCE THE INTESTINAL EPITHELIAL BARRIER

MARTÍNEZ MONTEROS, MARIA JOSE ; BALCELLS, MARÍA FLORENCIA; PERDIGÓN, GABRIELA; MALDONADO-GALDEANO, CAROLINA; CAZORLA, SILVIA INÉS

Congreso; LXVIII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); 2020

The epithelial layer not only serves as a passive physical barrier with external environment.Intestinal epithelial cells (IEC) sense external or endogenous danger signals and mount a robust immuneresponse to maintaining intestinal homeostasis. We previously demonstrated that lactobacillus increasePaneth cells and intestinal antimicrobial activity to keep this balance. Our aim was to furthercharacterized the effects of probiotics on the intestinal epithelial barrier (IEB). Mice were fed withLactobacillus casei CRL 431 (Lc431), Lactobacillus paracasei CNCM I- 1518 (Lp1518) for 7 and 5 days,respectively, or water. On day 8 mice were killed and samples were removed.Mice fed with the probiotics increased ex vivo intestinal antimicrobial activity againstStaphylococcus aureus, Salmonella thyphimurium and a multi-resistant drugs Klebsiella pneumoniae(p