PROBIOTIC LACTOBACILLUS: PROMISING WEAPON TO AMELIORATE DISORDERS ASSOCIATED TO INTESTINAL INFLAMATION

MARIA JOSE MARTÍNEZ MONTEROS; NOUR YAHFOUFI; MARIA FLORENCIA BALCELLS; GABRIELA PERDIGÓN; CAROLINA MALDONADO GALDEANO; SILVIA INES CAZORLA

San Miguel de Tucumán

Congreso; LXVII Reunión Científica Anual; 2019

Sociedad Argentina de Inmunología (SAI)

We have demonstrated that lactobacillus administration increases Paneth cells and intestinalantimicrobial peptides (AMPs) in healthy mice.Our aim was to explore whether probiotics can restore the AMPs production in intestinalinflammation. Mice were fed with: Lactobacillus casei CRL 431 (Group 1); Lactobacillusparacasei CNCM I- 1518 (Group 2) for 7 and 5 days, respectively, or water (Group 3 and 4).Intestinal inflammation was induced in G1, 2, and 3 by 7,5 mg/kg/day of indomethacin (I) onthe last 2 days. G4 received injections of PBS. On day 8 mice were killed and samples wereremoved.A significant body weight loss, anemia, shortening in the length of the small intestine andintestinal villi, mononuclear cells infiltration of the lamina propria and decrease in Paneth andGoblet cells were observed in G3 concerning control. Probiotics supplementation in intestinalinflammation induced only a slight modification of these parameters. Interestingly, theantimicrobial activity of the intestinal fluids of G1 and G2 against Salmonella Typhimurium,Staphylococcus aureus, and the multi-resistant drugs?: Klebsiella pneumoniae and Enterobacteraerogenes were higher regarding G3 and G4 (p