CONICET | Buscador de Institutos y Recursos Humanos

The thymus undergoes involution processes affecting its functionality caused by obesity andsenescence.Aim: to study the effects of the L. casei CRL431 strain or their cells walls on thymus recovery ina model of obesity and senescence mice.Methods: Male Balb/c mice were divided into groups according to the model: A) Obesity: wassubdivided according to diet/treatment: Normal Control (NC), NC+Probiotic (NC+P), ObeseControl (OC) and OC+Probiotic (O+P); B) Senescence: was subdivided according to treatmentand age: NC and NC+P: 21, 28, 45, 90 and 180 days (d). Body weight (BW) and thymus (T) ofboth models were taken. Samples were: large intestine (IG) for microbiota analysis (M), serum(S), small intestinal fluid (IF) and T for cytokine analysis. Cytokines determination in culturesupernatant of T stimulated with the probiotic bacteria (B) or its cell wall (W) by ELISA.Lymphocyte from T were analyzed by flow cytometry.Results: OC and OC+P reached the peak of BW in the ninth week and O+P decreased BW in 10thweek, in senescence weight gain was parallel to age; BW/T ratio increased in OC, NC28d andNC90d and decreased significantly in O+P. M showed probiotic administration managed tonormalize microbiota in both experimental models. CD4+ population decreased in OC, N+P45dand N+P90d respect to NC and increased in O+P; CD8+ population increased in OC, O+P, N+P90dand N+P180d respect to NC. Cytokine analysis showed an increase of IL-6, TNF-α, IL-12 anddecreased IL-10, IFN-γ and IL-3 in OC and as in the age increased; both oral administration withB and W can improve cytokine values.L. casei CRL431 showed to have beneficial effects on the thymus functionality in obesity andsenescence process with improvement of the status of the immunity.

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