EFFECT OF A PROBIOTIC STRAIN ON THYMUS T-LYMPHOCYTE POPULATION IN OBESE AND SENESCENT MICE MODELS

BALCELLS MARÍA FLORENCIA; MARTÍNEZ MONTEROS, MARÍA JOSÉ; CAZORLA, SILVIA INÉS; PERDIGÓN, GABRIELA; MALDONADO-GALDEANO, CAROLINA

Mar del Plata

Congreso; LXX REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); 2022

Sociedad Argentina de Inmunología

The thymus undergoes involution physiological processes that affect its functionality caused by senescence. The obesity can speed up this process with T lymphocytes population deterioration.Aim: to study the effect of a probiotic strain on the T population from the thymus of obese and senescent mice.Methods: Male Balb/c mice were divided in tow groups according to the experimental model: A) Obesity: was subdivided according to diet/treatment: Normal Control (NC), NC + Probiotic (NC+P), Obese Control (OC) and OC + probiotic (OC+P); B) Senescence: was subdivided according to treatment and age: NC and NC+P: 21, 28, 45, 90 and 180 days. A high fat diet was administered to obese mice group until they reached 25% more body weight compared to the NC group.Samples: Thymus (T) from obese and senescent mice were taken: T Lymphocyte (LT) were analyzed by flow cytometry and IL-7 was determined in culture supernatant of T stimulated with the probiotic bacteria (B) or its cell wall (W) by ELISA. Statistical analyses were performed by GraphPad Prism software using the ANOVA, with Tukey’s correction for multiple comparisons, p< 0.05 was considered statistically significant.Results: In both obesity and senescence mice, decreased LT-CD4+, LT-CD4+/CD8+ (DP) population and increased LT-CD8+ population were observed. Probiotic normalized LT-CD4+ and increased DP and LT-CD4-/CD8- (DN) compared to NC.IL-7 levels decreased significantly in both models (P