Animal Model for the Study of Acquired a-Manosidosis

CANCELARICH NATALIA LORENA; MARTINEZ AGUSTÍN; GARDNER DALE R.; ROBLES CARLOS A.; GIMENO EDUARDO J.; ROZENFELD PAULA A.

Medellin

Congreso; IX Congreso Latinoamericano de Errores Innatos del Metabolismo y Pesquisa Neonatal. SLEIMPN; 2013

Acquired a-manosidosis is caused by feeding of swainsoninecontainingplants. Swainsonine is an alkaloid that inhibits lysosomala-manosidasa. Swainsonine has been detected in plantsof genus Astragalus and causes intoxication. Different domesticanimals are susceptible to intoxication. Moreover, differentanimals, such as mouse, rat, and guinea pig, have been used asanimal models. Guinea pig model has been shown to be themost suitable because of higher sensitivity to the toxin.1 Theaim of this work is to characterize the pathology generatedby intoxication with plants from genus Astragalus, using guineapigs as the animal model. Guinea pigs were fed with Astragaluspehuenches-containing food (GP) or control food (GC).Clinical, biochemical, and histopathologic evaluation were performed.Clinically, activity of animals fed GP reduced whencompared with those fed GC. Excretion of oligosaccharide wasobserved in the GP group but not in GC animals in the firstweek and during whole course of the experiment. Changes inlysosomal a-manosidasa activity were not detected. Histologically,GP animals showed vaculation and cellular degenerationin central nervous system cells. These results suggest neuronalinjury in central nervous system caused by oligosaccharidedeposits, affecting the functionality.