B. abortus modulate osteoblast function through the induction of autophagy.

AYELÉN IVANA PESCE VIGLIETTI; PAULA CONSTANZA ARRIOLA BENITEZ; GUILLERMO HERNÁN GIAMBARTOLOMEI; MARÍA VICTORIA DELPINO

Mar del Plata

Congreso; LXI reunión anual de la sociedad argentina de investigación clínica (SAIC) LXIV Reunión anual de la sociedad argentina de inmunología (SAI) XLVIII Reunión anual de la sociedad argentina de farmacología experimental (SAFE).; 2016

Osteoarticular brucellosis is the most common localization of human active disease.Osteoblasts are specialized mesenchyme-derived cells involved in bone formation and are considered as professional mineralizing cells. We demonstrated that B. abortus infection modified osteoblast metabolism by the inhibition of the deposition of organic and mineral matrix, leading to bone loss. B. abortus replicative vacuole involved autophagy pathway activation and on the other hand, autophagy has been involved in osteoblast metabolism. Then experiments were conducted to determine if B. abortus modulates osteoblast function through the activation of autophagy. To this end, B.abortus infected osteoblasts cells (MC3T3 E1 cell line) were lysed to determine LC3II, beclin-1 and p62 expression by Western blotting. MMPs production was determined by gelatin zymography, collagen deposition by Sirius red staining and alizarin red S staining to determine calcium deposition. B. abortus infection increased the levels of LC3II (p