IRON OVERLOAD INDUCES CHANGES OF ZRT-IRT-LIKE PROTEIN 14 AND TRANSFERRIN RECEPTOR 1 TRANSPORTERS IN PANCREATIC CELLS IN MICE.

MARÍA FLORENCIA FERNÁNDEZ DELIAS; GIORGI GISELA; NORMA MARÍA GIUSTO; ROQUE MARTA ELENA

Mar del Plata

Congreso; .LXI Reunión Cientifica Anual de la Sociedad Argentina de Investigación Clínica. (SAIC) LXIV Reunión Anual de la Sociedad Argentina de Inmunología (SAI), XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental (SAFE), VII Reunión Anual; 2016

SAIC

We demonstrated that iron excess induce a coordinated expression of Hepcidin and of the divalent metal transporter1 (DMT1) iron importer in pancreatic tissue. However, the functions of other iron importers are not entirely clear in this tissue. Objective: The aim of this study was to clarify the effects of iron excess on pancreas, in terms of regulation of iron proteins such as transferrin receptor1 (TfR1) and ZIP14(Zrt-Irt-like Protein 14). Materials and Methods: CF1 female mice (25±5g; 3 months old) were bred at the animal facility of the UNS. After acclimation, mice were divided in two groups (n=6/group; paired design): 1)Iron adequate (FeA); 2)Iron overload (FeO) Fe-Saccharate (5d/20d.ip; 1,3g/kg). The procedures followed the Guide for the Care and Use of Laboratory Animals of NIH. The protocol was approved by the Committee on Experimental Animal Use and Care of the UNS. Immunohistochemical studies were assessed to determine TfR1, ZIP14 and Ferritin localization. Perl´s stain. Results: Slight TfR1 expression was observed in Langerhans islets in FeO, while abundant expression was observed in FeA. In acinar cells, we not detected TfR1 expression in both FeO and FeA mice. ZIP14 expression in acinar cells was intense in FeO and it was weak in FeA. However, in Langerhans islets, ZIP14 expression was similar for the two groups. The expression of ferritin detected in acinar and connective tissue cells was intense in FeO and it was slight in FeA. In Langerhans islets we observed weak ferritin expression in FeO, while it expression was intense in FeA. Abundant hemosiderin was observed in the pancreatic interacinar connective tissues in FeO, however it was absent in FeA. Conclusions: In iron overload, pancreatic ZIP14 might be the major transporter for iron excess uptake in acinar cells, while TfR1 seems not to be involved. Finally, we propose that pancreas possess an adaptive role in iron excess, which the exocrine area could protect the endocrine zone against iron.