FOSFOMYCIN PENETRATION INTO SWINE LEUKOCYTES

PÉREZ GAUDIO, DENISA SOLEDAD; MARTÍNEZ, GUADALUPE; FERNÁNDEZ PAGGI, MARÍA BELÉN; DECUNDO, JULIETA MARÍA; ROMANELLI, AGUSTINA; MOZO, JOAQUÍN; DIEGUEZ, SUSANA NELLY; SORACI, ALEJANDRO LUIS

INTERNATIONAL JOURNAL OF APPLIED RESEARCH IN VETERINARY MEDICINE

VETERINARY SOLUTIONS LLC

Año: 2018 vol. 16 p. 156 - 162

1559-4602

Fosfomycin (FOS) is an antibiotic widely used in Asia and Latin-America for the treatment and prevention of swine infections caused by resistant bacteria. Intracellular and interstitial fuids are biophases for facultative intracellular microorganisms. On the other hand, the choice of an antibiotic depends on its direct antimicrobial activity, which is in vitro determined by the MIC. FOS MIC90 ranges from 0.25 to 0.50 μg/mL for the most important pathogens in pig production. The aim of this research was to study the in vitro and in vivo FOS penetration into swine leukocytes (white blood cells-WBC) and to determine if intracellular concentrations exceed the MIC90 for most swine pathogens. For the in vivo, study six weaning piglets were intramuscularly administered a 15 mg/kg dose of disodium FOS. For the in vitro study, WBC obtained from weaning piglets were incubated with a 150 μg/mL dose of disodium FOS. Intracellular concentrations were analyzed by HPLC MS/MS and they ranged from 0.89 to 3.88 μg/mL for the in vitro assay (Tmax: 4 h) and between 0.16 and 1.33 μg/mL for the in vivo study (Tmax: 0.5 h). The values found in both studies are above the MIC90 for the most important pathogens in swine production. These values were maintained for more than 24 h for the in vitro study (T> MIC=24h) and for more than 4 h for the in vivo study (T> MIC=4 h).