Desarrollo de nanomateriales magnéticos biocompatibles basados en hidroxiapatita con potenciales aplicaciones biológicas

DITTLER, M. L.; DELL'ARCCIPRETE, M. L.; RIVERO BERTI, I.; GONZÁLEZ, M.

Santa Fe

Congreso; CONGRESO INTERNACIONAL DE METALURGIA Y MATERIALES SAM-CONAMET/IBEROMAT/MATERIA 2014; 2014

SAM-CONAMET/IBEROMAT/MATERIA

The use of superparamagnetic nanomaterials in medical and biological applications increases in last decades due to its special properties in drug delivery, hyperthermia, imaging, etc. However, the low biocompatibility of these materials diminishes the therapeutic use. One of the strategies to solve the difficulty has been the synthesis of a bioactive material, Hydroxyapatite, HAp, main bone component, doped with iron (FeHAp). The practice limits the magnetic low-biotolerance phase formation. Thus, it is intended to obtain a material which can satisfy the increased demand on regenerative tissue medicine and drug delivery systems The aim of this work is to develop hydroxyapatite based nanomaterials with magnetic properties, functionalize these materials with biomolecules to promote cell adhesion, characterize the materials and finally used them to coat liposomes that will act as drug delivery systems. The nanomaterials were synthesized by dripping method and simultaneous addition of salts of Fe (II) and Fe (III). Then, they were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) spectroscopy X-ray photoelectron (XPS), infrared spectroscopy with attenuated total reflectance (FTIR-ATR), Thermogravimetric Analysis, Fluorescence, Adsorption Isotherms, Magnetization Curves, Dynamic Light Scattering and Z Potential. Results The FeHAp synthesis temperature was optimized at 80ºC. TEM images showed the formation of cane-type nanostructures and a high degree of aggregation. DLS results also showed a monodispersed distribution and the formation of aggregates nanostructures in the micron scale. The magnetization results revealed that obtained materials present superparamagnetic properties. A FeHAp surface composition of Ca1P0.85O4.7Fe0.19 was estimated from XPS measurements. The addition of Acridine Orange to the coated liposomes was confirmed by stationary fluorescence measurements.