LPS FROM PORPHYROMONAS GINGIVALIS IMPAIRS TROPHOBLAST CELL FUNCTION AND IMMUNE-TROPHOBLAST INTERACTION

HAUK, VANESA C.; CALO, GUILLERMINA; ROMÁN MUÑOZ, MARÍA; VOTA, DAIANA M.; MERECH, FÁTIMA; PAPARINI, DANIEL E.; RAMHORST, ROSANNA; PÉREZ LEIRÓS, CLAUDIA

Congreso; IFPA; 2019

Objectives: Porphyromonas gingivalis (Pg), an important pathogen of periodontal disease, has been implicated in adverse pregnancy outcome although the mechanisms involved are still unclear. Lipopolysaccharide from Porphyromonas gingivalis (Pg-LPS), the main virulence factor of Pg, differs from other bacterial LPS in its structure and function: due to the presence of lipoproteins, Pg-LPS activates not only TLR4 but also TLR2 mediated pathways. The aim of this study was to examine the effect of Pg-LPS on trophoblast cell function and trophoblast-neutrophil interaction and to explore TLR4/TLR2 mediated mechanisms. Methods: Swan-71 human trophoblastic cell line was treated with PgLPS (10ng/ml). Cytokine and chemokine expression was evaluated by RTqPCR, glucose uptake by flow cytometry using the fluorescent analogue 2-NBDG and cell invasion assessed in Matrigel-covered transwells Peripheral blood neutrophils were purified from healthy donors and cultured with conditioned media of trophoblast cells (TbCM) treated or not with LPS (PgLPS-CM); apoptosis was determined by fluorescence microscopy and CD11b and reactive oxygen species (ROS) were evaluated by flow cytometry. Results: Pg-LPS altered trophoblast function: It reduced cell invasion, impaired pro-inflammatory/ antiinflamatory cytokine expression and decreased glucose uptake (P