VIP enhances apoptotic cell phagocytosis by monocyte/macrophages in an in vitro model of immune-trophoblast interaction

PAPARINI, DANIEL; GRASSO, ESTEBAN; CALO, GUILLERMINA; VOTA, DAIANA; LAURICELLA, ANA MARÍA; QUINTANA, IRENE; RAMHORST, ROSANNA; PÉREZ LEIRÓS, CLAUDIA

Mar del Plata

Simposio; VI SLIMP V LASRI; 2015

Latin American Society for Maternal Fetal Interaction and Placenta

PA.24 Homeostasis maintenance at the early maternal-fetal interface requires the clearance of apoptotic cells by macrophages bearing an immunosuppressant profile. VIP is a pleiotropic polypeptide synthesized by trophoblast cells that promotes an anti-inflammatory profile in monocytes (Mo) and macrophages (Ma), however, its role to modulate macrophages at early pregnancy has not been elucidated yet. Objective To explore VIP effect on trophoblast-monocyte/macrophage interaction with special focus on Mo/Ma migration, phenotype and phagocytosis of apoptotic cells. Methods Mo were purified by Percoll from blood mononuclear cells of healthy women and cultured with conditioned media (CM) from a first trimester human cytotrophoblast cell line (Swan-71, Tb) in the presence/absence of VIP (10-100 nM). Apoptosis was induced in Tb with camptothecin. CFSE-labelled apoptotic cell phagocytosis by CD14+ cells and migration through 5um pore transwells was determined by FACS. Expression of CD39, IL-10, IL-12, TNF-α and adhesion molecules was also evaluated by FACS. Thrombospondin expression was determined by RT-PCR. Results CM from Tb cells treated for 18 h with VIP (CM-VIP) increased CD14+ cell migration to a higher extent than CM alone (p