VIP enhances trophoblast cell-mediated monocyte migration, phagocytosis and M2 phenotype expression

PAPARINI, DANIEL; GRASSO, ESTEBAN; CALO, GUILLERMINA; VOTA, DAIANA; RAMHORST, ROSANNA; PÉREZ LEIRÓS, CLAUDIA

Mar del Plata

Congreso; LIX Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC) LXII Reunión Anual de la Sociedad Argentina de Inmunología (SAI); 2014

SAIC-SAI

The generation and maintenance of the maternal-placental interface is a dynamic process that involves the concerted action of different population of leucocytes and mediators to favor a tolerogenic micro-environment. Monocytes (Mo) are recruited to the maternal-placental interface and ?educated? by trophoblast cells (Tb) to express an M2 alternative activation profile, thus contributing to the maintenance of immune homeostasis. On the basis that vasoactive intestinal peptide (VIP) has anti-inflammatory and immunosuppressive effects, we evaluated the functional profile of Mo co-cultured with Tb and their modulation by VIP. We used an in vitro model of co-culture of first trimester trophoblast cell line (Swan-71) and Mo isolated from PBMCs (Percoll gradient) from healthy volunteers. The expression of cytokines and VIP receptors (VPAC) was evaluated in Mo by FACS and RT-PCR. Mo treated with VIP increased the % of CD39 while diminished IL-12 and TNF-a positive cells. When Mo were co-cultured with Tb alone or in the presence of 10 nM VIP the % of IL-10 positive cells was enhanced (X±ES= 29,5±3,5; 32,4±4,3 vs 16,3±4,3; P