Functional profile of Monocytes/Macrophages in the interaction with trophoblast cells and their modulation by VIP

PAPARINI, DANIEL; GRASSO, ESTEBAN; CALO, GUILLERMINA; BARBANO WEINGRILL, RODRIGO; CORREA, SIMONE; BEVILACQUA, ESTELA; RAMHORST, ROSANNA; PÉREZ LEIRÓS, CLAUDIA

Córdoba

Congreso; LXI Reunión Anual de La Sociedad Argentina de Inmunología; 2013

Sociedad Argentina de Inmunología

The generation and maintenance of the maternal-placental interface is a dynamic process that involves the concerted action of different population of leucocytes and mediators to favor a tolerogenic micro-environment. Monocytes (Mo) are recruited to the maternal-placental interface and ?educated? by trophoblast cells (Tb) to express an M2 alternative activation profile, thus contributing to the maintenance of immune homeostasis. On the basis that vasoactive intestinal peptide (VIP) has anti-inflammatory and immunosuppressive effects, we decided to evaluate the functional profile of Mo/Macrophages (Mo/Ma) co-cultured with Tb and their modulation by VIP. We used an in vitro model of coculture of first trimester trophoblast cell lines (Swan-71) and Mo or Ma isolated from PBMCs (Percoll gradient) or from third trimester term decidual tissue (immunomagnetics beads) from healthy volunteers. The expression of cytokines and VIP receptors (VPAC) was evaluated in Mo by RT-PCR and ELISA. Mo cocultured with Tb or conditioned media (CM) increased the secretion of IL-10 (X±SE: 139±23 vs 85±6 pg/ml, P