CHEMOKINES MEDIATED INTERACTION BETWEEN MATERNAL MONOCYTES AND TROPHOBLAST CELLS IS DIFFERENTIALY INDUCED BY PAMPs STIMULI

GRASSO, ESTEBAN; PAPARINI, DANIEL; FRACCAROLI, LAURA; HAUK, VANESA; SALAMONE, GABRIELA; PÉREZ LEIRÓS, CLAUDIA; RAMHORST, ROSANNA

Foz de Iguazu

Simposio; V Latin American Symposium on Maternal-Fetal Interaction and Placenta and IV Latin American Symposium on Reproductive Immunology; 2013

SLIMP-CLA ASRI

Background: We investigated chemokine/chemokine receptor modulation on monocytes during their interaction with trophoblast cells; and whether PAMPs stimuli modulate their functional profile and migratory properties. Methods: Swan 71 cells were cultured with monocytes isolated from fertile women in transwell sytems that allow or not monocyte migration. Trophoblast cells were challenged with LPS, PGN or poly[I:C]. Supernatants and cells were recovered for ELISA, FACS and RT-PCR analysis. (Protocol aproved Academy of Medicine Ethics Committee). Results: Maternal CD14+ cells increased IL-10 while decreased IL-1 and TNF production after the interaction with Swan-71 cells. Monocyte migration toward trophoblast cells was not increased in the presence of LPS or PGN as we observed with poly[I:C], and was accompained by a decreased expression of CCR1, CCR5, CXCL-8 and CCL5. Trophoblast cells did not increase chemokine expression after cultured with monocytes in the presence of pathological stimuli. Conclusion: Trophoblast might induce an alternative macrophage profile and restrain maternal monocyte recruitment under pathological stimulation.