Sexual dimorphism in the immunometabolic profile of Hofbauer cells

PAPARINI, DANIEL E.; GRASSO, ESTEBAN; AGUILERA, FRANCO; LARA, BRENDA; ARSLANIAN, M. AGUSTINA; LELLA, VICTORIA; HAUK, VANESA; MERECH, FÁTIMA; SCHUSTER, CLAUDIO; GORI, SOLEDAD; MELLER, CESAR; MARTÍ, MARCELO A.; RAMHORST, ROSANNA; VOTA, DAIANA; PÉREZ LEIRÓS, CLAUDIA

San Luis

Congreso; LXXI REUNION ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGIA (SAI); 2023

SAI

Aims: Hofbauer cells (HB) are the only foetal immune cell population within the stroma of healthy placenta. However, the functional properties of these cells are poorly described. HB are transcriptional and chromosomically similar to yolk sac macrophages. Here we studied the effect of sexual dimorphism in the immunometabolism of human placental HB at term.Methodology: HB cells were obtained from placental tissue by enzymatic digestion from healthy pregnant women at term along with autologous PBMCs. HB were cultured overnight and the phenotypic profile, efferocytosis, glucose uptake and lipid droplets accumulation were evaluated by flow cytometry and ELISA. We have started with bioinformatic analysis in public database to understand the differential imprinting of placentas with male or female newborns.Results: HB from female placenta (fHB) present higher production of antiinflammatory markers CD163, CD206 and CD39 accompanied by lesser secretion of IL-1B than HB from male placentas (mHB) (*P