MATERNAL IMMUNE CELLS TRANSFERRED THROUGH COLOSTRUM DO NOT INTERFERE WITH THE IMMUNE RESPONSES TO FOOT-AND-MOUTH DISEASE (FMD) VACCINE

BUCAFUSCO, DANILO; DI GIáCOMO, SEBASTIáN; RODRIGO PEREYRA; PEGA, JUAN; MALACARI, DARíO; JUNCOS, SOL; SCHAMMAS, JUAN MANUEL; PéREZ-FILGUERIA, MARIANO; CAPOZZO, ALEJANDRA

Catvat

Workshop; Open Session of the Standing Technical Committee; 2014

EU-FMD

Introduction: Little is known on the influence of maternal antibodies and cells transferred through colostrum in the immune responses of calves to the currently used FMD vaccines. In this study, we evaluated the humoral and cellular immune responses induced by vaccination of colostrum-deprived calves and of calves that received equivalent amounts of colostrum preparations that differed in the presence or absence of maternal immune cells but were equivalent in terms of quantity and quality of anti-foot-and-mouth disease virus (FMDV) antibodies. Materials and methods: The current oil-adjuvanted commercial tetravalent vaccine used in Argentina was applied to 30 days-old calves that were deprived of colostrum (n=3), fed with whole immune-colostrum (WC) from their mothers (n=4 ) or with a cell-free colostrum, containing only anti FMDV antibodies (n=3). The animals were bled periodically before vaccination, and weekly after vaccination. Immune responses were determined in terms of T-cell proliferation, IFNg production against all vaccine strains, total and neutralizing antibodies, avidity and isotypes of anti O1/Campos antibodies. Results. Anti-FMDV cell-mediated immune responses were detected in non-vaccinated calves that received WC. All vaccinated calves developed IFN-ã and lymphoproliferative responses, irrespectively of the colostrum received. Colostrum-deprived animals responded to vaccination with a primary IgM response (14 dpv) followed by an increase of IgG1 titers, a profile comparable to that obtained in adult animals. Conversely, all colostred calves showed a decrease in antibody titres after vaccination, following the decay curve for maternal antibodies. The higher the initial antibody titer, the greater the fall in titres observed after vaccination. Conclusions: This study demonstrates for the first time that maternal immune cells transferred to the calves though colostrum do not modify the immune responses to FMD vaccine, and confirms the interference of maternal antibodies in the induction of humoral responses in colostred calves. Introduction: Little is known on the influence of maternal antibodies and cells transferred through colostrum in the immune responses of calves to the currently used FMD vaccines. In this study, we evaluated the humoral and cellular immune responses induced by vaccination of colostrum-deprived calves and of calves that received equivalent amounts of colostrum preparations that differed in the presence or absence of maternal immune cells but were equivalent in terms of quantity and quality of anti-foot-and-mouth disease virus (FMDV) antibodies. Materials and methods: The current oil-adjuvanted commercial tetravalent vaccine used in Argentina was applied to 30 days-old calves that were deprived of colostrum (n=3), fed with whole immune-colostrum (WC) from their mothers (n=4 ) or with a cell-free colostrum, containing only anti FMDV antibodies (n=3). The animals were bled periodically before vaccination, and weekly after vaccination. Immune responses were determined in terms of T-cell proliferation, IFNg production against all vaccine strains, total and neutralizing antibodies, avidity and isotypes of anti O1/Campos antibodies. Results. Anti-FMDV cell-mediated immune responses were detected in non-vaccinated calves that received WC. All vaccinated calves developed IFN-ã and lymphoproliferative responses, irrespectively of the colostrum received. Colostrum-deprived animals responded to vaccination with a primary IgM response (14 dpv) followed by an increase of IgG1 titers, a profile comparable to that obtained in adult animals. Conversely, all colostred calves showed a decrease in antibody titres after vaccination, following the decay curve for maternal antibodies. The higher the initial antibody titer, the greater the fall in titres observed after vaccination. Conclusions: This study demonstrates for the first time that maternal immune cells transferred to the calves though colostrum do not modify the immune responses to FMD vaccine, and confirms the interference of maternal antibodies in the induction of humoral responses in colostred calves.