DEVELOPMENT OF IN HOUSE OPEN AND MODULAR RT-qPCR PROTOCOL FOR SIMULTANEOUS DETECTION OF RESPIRATORY VIRUSES

CARRILLO ANGELES; BRAECKMAN CARLOS; MANZUR MARÍA JIMENA; JURI AYUB MAXIMILIANO

Mendoza

Congreso; XL Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2022

DEVELOPMENT OF IN HOUSE OPEN AND MODULAR RT-qPCR PROTOCOL FORSIMULTANEOUS DETECTION OF RESPIRATORY VIRUSESCarrillo A 1 , Braeckman C 2 , Manzur J 1 , Juri Ayub M 1 (*)1 Laboratorio de Desarrollo de Diagnósticos Moleculares, Área Biología Molecular. FQByF. UniversidadNacional de San Luis. 2 Laboratorio BioMAv, San Luis . (* Contacto: mjuriayub@hotmail.comDuring the pandemic COVID 19, social distancing required for avoiding the collapse of sanitary systemsdrastically reduced the circulation of respiratory viruses other than SARS Cov2. Currently, due to the successfulof massive vaccination campaign, social connectivity is virtually at pre pandemic levels. Therefore, in additionto SARS Cov2, other respiratory viruses are currently circulating in the population. Therefore, simple and costeffective tools for diagnostic and epidemiology of respiratory pathogenes are desirable. In our lab, we havemodified, adapted and optimized previously validated singleplex RT-qPCR assays in a multiplex format. In thisway, we developed a cuádruplex assay detecting and identifying three pathogens; namely SARS Cov 2,Influenza A and Sincitial Respiratory Virus, along with a human gen as saimple integrity control. Thecuádruplex reaction was able to detect every virus in anonimized samples, even when mixed infections weresimulated.