CHEMICAL COMPOSITION AND ANTIBACTERIAL ACTIVITY AGAINST Staphylococcus aureus ATCC 25923 OF THE ESSENTIAL OILS FROM Baccharis spartioides ("Pichana") EXTRACTED BY STEAM DISTILLATION

MANSO V.; LAZZARINI NATALIA; ANDREA COSTANTINO; SCARONE N.; MOLDES C. A; BELLOZAS R.N; MINIG M.

San Luis - Argentina

Encuentro; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2019

Sociedad de Biologia de Cuyo

B. spartioides is an plant aromatic member of the Asteraceae family. Its branches are strongly scent because presence of wide variety of volatile compounds which they have demonstrate antimicrobial activity. The present work aims to determine essential oil composition of B. spartioides (BsEO) and assess the antimicrobial activity against S. aureus. BsEO was obtain by steam distillation, and its composition was determine by GC-MS. The antimicrobial activity was evaluated by disc diffusion test which diameter of the clear zone around the disc was measured and expressed in millimeters. It was assess the antimicrobial activity by increasing volumes of BsEO impregnated on disc using 6 μL of pure limonene (97%) and Amikasin (30 μg) as discs control. It was considerate diameters >20 mm by significant inhibitory effect. The majors constituents of BsEO were limonene (44%), sphatulenol (12%) and caryophyllene oxide (8%). Since limonene is BsEO major constituent, we considered this component as responsible of antimicrobial activity. Thus, calibration curve of μL of limonene vs diameter was made and it was determined that 6 μL of BsEO was the minimal volume for significant inhibitory effects. Major antimicrobial activity was expressed in discs impregnate with 5, 6, 7 and 8 μL of BsEO, which diameters was inferior to 20 mm. Results indicate BsEO present low antimicrobial activity (