INVESTIGADORES
SANZ BLASCO Sara Isabel
artículos
Título:
17beta-estradiol promotes cementoblast proliferation and cementum formation in experimental periodontitis
Autor/es:
NÚÑEZ J; SANZ BLASCO S; VIGNOLETTI F; MUÑOZ F; CAFFESSE RG; SANZ M; VILLALOBOS C; NUÑEZ L
Revista:
JOURNAL OF PERIODONTOLOGY
Editorial:
AMER ACAD PERIODONTOLOGY
Referencias:
Año: 2010 vol. 81 p. 1064 - 1074
ISSN:
0022-3492
Resumen:
Background:
Periodontitis is a chronic inflammatory condition that leads to the
destruction of the tooth-supporting tissues. Its treatment includes the
arrest of the inflammatory process and, in some circumstances, the
restoration of the lost anatomy and function, including the formation of
new cementum, periodontal ligament (PDL), and bone. With this goal, we
investigated the effects of low concentrations of 17β-estradiol on human
cementoblast proliferation and its possible regenerative potential in
vivo.
Methods: Human cementum-derived cells obtained from a
healthy human premolar were isolated and characterized by
immunocytochemistry. Cell proliferation assays were performed to test
the effects of 100 nM 17β-estradiol and enamel matrix derivative (EMD).
Three-wall intrabony periodontal defects were created in beagle dogs.
After 1 month of plaque accumulation, 0.225 mg 17β-estradiol impregnated
in a collagen sponge was applied to randomly selected defects (test
group), whereas a collagen sponge impregnated in a culture medium was
applied to the control group. After 3 months, specimens were obtained,
and tissue regeneration was assessed by histometric analysis.
Results:
Cells spreading out from human tooth-layer explants were able to form
cell colonies, produce a mineral matrix, and express osteocalcin,
indicating they were cementoblast-like cells. In contrast, PDL
fibroblasts did not express osteocalcin. 17β-estradiol, but not EMD,
increased the rate of human cementoblast cell proliferation in vitro by
2.5-fold. Histometric results from the treated periodontal defects
revealed that 17β-estradiol promoted the formation of 2.94 mm of new
cementum, (45% of the defects) compared to 1.54 mm of new cementum in
the control group (28% of the defects). Furthermore, the test group
showed an inhibition of epithelial downgrowth and a gain of new
connective tissue attachment.
Conclusion:
17β-estradiol promoted human cementoblast cell proliferation in vitro
and periodontal regeneration in an experimental periodontitis model.