INVESTIGADORES
SANZ BLASCO Sara Isabel
artículos
Título:
Periodontal regeneration following implantation of cementum and periodontal ligament-derived cells.
Autor/es:
NÚÑEZ J; SANZ BLASCO S; VIGNOLETTI F; MUÑOZ F; ARZATE H; VILLALOBOS C; NUÑEZ L; CAFFESSE RG; SANZ M
Revista:
JOURNAL OF PERIODONTAL RESEARCH
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2011 vol. 47 p. 33 - 44
ISSN:
0022-3484
Resumen:
Background and Objective: The periodontal
regeneration of bone defects is often unsatisfactory and could be
largely improved by cell therapy. Therefore, the purpose of this study
was to evaluate the regenerative potential of implanting canine
cementum-derived cells (CDCs) and canine periodontal ligament-derived
cells (PDLDCs) in experimentally created periodontal intrabony defects
in beagle dogs.
Material and Methods:
Cells were obtained from premolars extracted from four beagle dogs.
Three-wall intrabony periodontal defects, 3 mm wide and 4 mm deep, were
surgically created in their second and fourth premolars and plaque was
allowed to accumulate. Once the defects were surgically debrided,
periodontal regeneration was attempted by random implantation of
collagen sponges embedded with 750,000 CDCs, 750,000 PDLDCs or culture
medium. After 3 mo of healing, specimens were obtained and periodontal
regenerative outcomes were assessed histologically and histometrically.
Results:
The histological analysis showed that a minimal amount of new cementum
was formed in the control group (1.56 ± 0.39 mm), whereas in both test
groups, significantly higher amounts of new cementum were formed
(3.98 ± 0.59 mm in the CDC group and 4.07 ± 0.97 mm in the PDLDC group).
The test groups also demonstrated a larger dimension of new connective
tissue, resulting in a significantly more coronal level of histological
attachment.
Conclusion: This
proof-of-principle study suggests that cellular therapy, in combination
with a collagen sponge, promoted periodontal regeneration in
experimental intrabony periodontal defects.