Pathogen-specific T cells from Patients with Inflammatory Bowel Diseases can be Modulated by Probiotics from Kefir

RENATA CURCIARELLO; SANTIAGO BRAYER; GUILLERMO DOCENA; AUGUSTO VILLAVERDE; FRANCISCO TUFARÉ; LAURA GARBI; GUSTAVO CORREA; MARTIN YANTORNO; ALICIA SAMBUELLI; ANDRES ROCCA; KARINA CANZIANI; AYELEN HUGO; CECILIA ALVAREZ; MARTÍN RUMBO; AGUSTINA ERREA; MARÍA DE LOS ANGELES SERRADELL; EMANUEL BARBIERA; ILEANA SALTO; CECILIA MUGLIA

Congreso; Reunion conjunta SAIC SAI SAFIS 2018; 2018

SAIC SAI SAFIS

Kefir is a fermented milk with health-promoting properties. Laminapropria T cells (LPTC) play a central role in the pathogenesis of InflammatoryBowel Diseases (IBD). These cells contribute to mucosalinflammation by secreting pro-inflammatory cytokines and beingresistant to apoptosis. We aimed to modulate the proliferation andthe pro-inflammatory response of pathogen-specific LPTC from IBDpatients using microorganisms from kefir.Colonic biopsies from IBD patients were incubated with probioticsfrom kefir and cytokines in supernatants were evaluated by ELISAafterwards. LPTC were isolated from biopsies (N=23; 7 from Crohn?sDisease patients-CD and 15 from ulcerative colitis-UC patients) bycollagenolytic digestion. In order to expand pathogen-specific T lymphocytes,cells were cultured with enteroadhesive (EA) Escherichiacoli extract and IL-2 for 10 days. Thereafter, LPTC were incubatedwith anti-CD3/anti-CD28 and with microorganisms from kefir (Enterococcusdurans and Lactobacillus kefiri), their conditioned mediaor 10 μM lactate for 96 hs. LPTCs proliferation (CFSE) and TNF-α,IFN-γ, IL-6, IL-10 and IL-13 cytokine secretion were evaluated (ELISA).We found that E. durans and L. kefiri diminished the pro-inflammatorycytokine production by inflamed tissues (IL-6: 1800±670 and576±3 vs 6900±2000 pg/ml basal production, respectively; IL-8:38000±11000 and 32000±7300 vs 80000±27000 pg/ml basal production,respectively). EA E. coli specific LPTC lines were developedfrom all patients. Cellular proliferation of activated CD4+/CFSE-/PILPTCdecreased significantly with L. kefiri , E. durans and lactate(P