Increased content of Lysophospholipids, diacylglicerides and sphingomyelins in isolated triglycerides-rich lipoproteins and HDL from patients with coronary artery disease.

BARCHUK, MAGALI; SVILAR, LJUBICA; ANCEL, PATRICIA; MIKSZTOWICZ, VERONICA; LOPEZ GRACIELA; RUBIO, MIGUEL; LAURA SCHREIER; DUTOUR, ANNE; GABORIT, BÉNÉDICTE; MARTIN, JEAN CHARLES; BERG, GABRIELA

Clermont-Ferrand

Jornada; 12émes journées scientifiques du Reseau Francaise de Metabolomique et Fluxomique,; 2019

Introduction: android obesity and the atherogenic dyslipidemia, with increased TG-rich lipoproteins remnants (TRL) and decreased HDL levels, are associated with higher risk of coronary artery disease (CAD). Plasma lipidomicsstudies have revealed that particularmolecular lipid species are involved in the development of atherosclerosis and CAD. However, the lipidomic analysis of isolated lipoproteins (TRL and HDL)has been scarcely studied so far. This qualitative analysis is of interest as it would allow differentiating specific lipid speciescontributiontothe development of CAD. Materials and Methods: lipidomic analysis was performed in15 patients undergoing coronary artery bypass graft (CAD) and 12 patients with valve replacement (No CAD). Serum samples were obtained before surgery and TRL and HDL were isolated by ultracentrifugation. For lipidomics, lipids were extracted from each fraction with MTBE. Lipidomics was carried out on a LCC18 column hyphenated to a Q-Exactive plus mass spectrometer, using both positive and negative ionization mode, and acquired in full MS and data dependent MSMS mode. Lipid annotation was performed using a combination of the open source XCMS and ThermoFischerLipidSearch software.Results: a total of 242 lipid species were identified in both lipoproteins fractions. In CAD patients, higher content of total LPC (p