Is Endothelial Lipase a supporting actor of Lipoprotein Lipase? Study from the gene to the activity in an obesity model.

MAGALÍ BARCHUK ; VERÓNICA MIKSZTOWICZ; AGATA CEVEY; VALERIA ZAGO; GRACIELA LÓPEZ; ROSARIO MECOZZI; SILVIA FRIEDMAN; NORA GOREN; CELINA MORALES; LAURA SCHREIER; GABRIELA BERG

Congreso; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2018

Lipoprotein lipase (LPL) and endothelial lipase (EL) are involved in lipoproteins metabolism. Ininsulin-resistance (IR), with visceral adipose tissue (AT) expansion and cardiac steatosis, thisenzymesbehavior is altered. Peroxisome Proliferator Activated Receptors (PPARs) and apoproteins (apo)CII andCIII could be partly responsible for these alterations. Aim: evaluate LPL and EL mRNA, protein levelsand activity in AT and heart, association with lipoprotein profile and the role of PPARs and apoCs in anobesity model.Methods: Male Wistar rats were fed with standard diet (Control,n=14) or High Fat Diet (HFD,n=14)during 14 weeks. Glucose, lipoprotein profile and IR markers were measured. Histological studies wereperformed in heart and epididymal AT. LPL and EL mRNA levels were assessed by RT-qPCR, proteinslevels by Western Blot and enzymes activities by radiometric assays. Cardiac and AT PPARsexpression were measured by Western Blot and hepatic apoCs mRNA by RT-qPCR.The study wasapproved by the CICUAL-FFYB(UBA).Results:In HFD, fat depots were observed in hearts, whereas AT presented higher adipocyte size. Inheart and AT, no differences were found in EL mRNA levels between groups, while AT LPL mRNAand protein levels were decreased in HFD (p=0.04 and p=0.01,respectively), without differences inheart. In both tissues, EL protein levels and activity were increased (p=0.05 and p=0.001,respectively)and inversely associated with decreased LPL activity (heart: R=-0.64,p