THE ROLE OF THIOREDOXIN 1 IN THE ISCHEMIC POSTCONDITIONING ON MICE FED HIGH FAT DIET

MAZO, TAMARA; GOMEZ, ANABELLA; ZAOBORNYJ, TAMARA; PEREZ V; CICALE E; CASANOVA, V; BARCHUK, MAGALI; BERG GABRIELA; D´ANNUNZIO, VERONICA; GELPI R

buenos aires

Congreso; Reunion Conjunta de sociedades de Biociencias; 2017

Sociedades de biociencias

Thioredoxin-1 (Trx1) has cardioprotective effects against ischemia/reperfusion (I/R) injury, and participates in mechanisms mediatingischemic postconditioning (PostC). It remains unclear whether the PostC protective effects are exerted during initial stages of atheroscleroticdisease by alteringTrx1 expression. The objective of thisstudy was to generate a murine high-fat diet (HFD) fed model thatdeveloped phenotypes consistent with early-stage atherosclerosis,and then evaluate increased oxidative stress, altered mitochondrialbioenergetics and Trx1 expression in the HFD model. Ultimately, themodel was used to determine whether HFD exposure abolished thecardioprotection conferred by PostC. C57/BL6 mice were fed withcontrol diet (CD) or HFD for 12 weeks. Mice isolated hearts was subjectedto 30 min of ischemia and 120 min of reperfusion (I/R group).For PostC subjects, after ischemia, six cycles were performed ofreperfusion/ischemia (10 sec respectively, per cycle) at the onsetof reperfusion. Data are expressed as mean±SEM and p