CATALASE AND SUPEROXIDE DISMUTASE BEHAVIOR IN EPICARDIAL ADIPOSE TISSUE FROM PATIENTS WITH CORONARY ARTERY DISEASE

MIKSZTOWICZ, VERONICA; REPETTO, ESTEBAN; BARCHUK, MAGALI; LOPEZ, GRACIELA; BALDI, J; CYMERYNG C; SCHREIER , LAURA; RUBIO, MIGUEL; BERG, GABRIELA

Mar del Plata

Congreso; LXI Reunion Cientifica Anual de la Sociedad Argentina de Investigacion Clinica; 2016

SAIC

Epicardial adipose tissue (EAT) is a visceral fat adjacentto coronary arteries and myocardium. EAT is considered anendocrine organ, and its volume an independent risk factor forcoronary artery disease (CAD). Catalase (CAT) and SuperoxideDismutase (SOD) are antioxidant enzymes that protect cellsagainst potentially harmful effects of reactive oxygen species.Until now, there is no evidence about the behavior of theseenzymes in EAT from patients with CAD. Aim: to evaluate CATand SOD activity in EAT from patients with CAD and their relationshipwith insulin-resistance (IR). Subjects and Methods:Peripheral venous blood, EAT and subcutaneous adipose tissue(SAT) were obtained from patients undergoing heart surgery forcoronary artery bypass graft (CAD, N=10) or valve replacement(No CAD, N=10). In serum glucose, lipids and lipoproteins profi lewas assessed. In EAT and SAT, CAT activity was evaluated bydecomposition of H2O2 at 240 nm, and SOD activity accordingto pyrogallol autoxidation method at 420 nm. The study wasapproved by the Ethic Committee of the Hospital de Clínicas,UBA. Results: In both groups there were no differences in age,body mass index, glucose or lipoprotein profile. In EAT fromCAD patients, SOD activity was decreased compared to No CAD(p=0.024) without differences in CAT activity. SOD and CAT activitieswere not associated with lipoprotein profile, however bothenzymes showed a negative correlation with TG/HDL-chol as IRmarker (p=0.01). Moreover, in EAT, CAT activity was decreasedin patients with TG/HDL-chol ≥3 (p=0.038). In both groups, nodifferences in SOD and CAT activity was found between EATand SAT. Conclusions: this is the first time that CAT and SODactivities are reported in EAT. Our findings propose a possibleregulation of these enzymes by IR in EAT, suggesting an increasein oxidative stress in this tissue; future studies are necessary toexplain the regulation of both enzymes.