Sampling and processing blood samples within the South American Youth/Child cARdiovascular and Environmental (SAYCARE) Study

LÓPEZ, GRACIELA; ASSALI SERRUYA, RAPHAEL; BARCHUK, MAGALÍ; GAITAN-CHARRY, DIEGO; TORRES-LEAL, FRANCISCO LEONARDO; MORENO, LUIS ALBERTO; DELGADO, CARLOS ALBERTO; BARBOSA CARVALHO, HERÁCLITO; DE MORAES, AUGUSTO CÉSAR FERREIRA; BERG, GABRIELA

Scientific Reports

Nature Publishing Group

Año: 2020 vol. 10 p. 1 - 7

Obesity and overweight in children and adolescents is increasing rapidly worldwide; however, scarcedata have been reported from South America countries. With the purpose of assessing hyperlipidemia,insulin resistance and chronic infammation, the evaluation of blood biomarkers such as glucose,lipoproteins and chronic infammation proteins is required. In the context of the SAYCARE study, inchildren and adolescents (3 to 18 years) from seven South American cities, our aim was to assess theimpact of pre analytical conditions on diferent biomarkers evaluated in 474 fresh serum samples, indiferent country centers. We also evaluated the stability according to time and frozen storage withinthis study across the concordance of the results obtained from the 49 blood samples measured in threediferent centers. Signifcant correlations as well as concordance were observed in TG, Total-C, HDL-Cand glucose between Buenos Aires and São Paulo. The samples evaluated in Teresina and São Paulopresented similar results, with exception of total cholesterol. We observed acceptable concordancebetween Buenos Aires vs São Paulo and Teresina vs São Paulo, suggesting that samples could beprocessed in each of these centers. This concordance is a consequence of the strict pre analyticalconditions previously established in the SAYCARE study