INVESTIGADORES
BERG Gabriela Alicia
artículos
Título:
Measurement of fasting salivary insulin and its relationship with serum insulin in children
Autor/es:
BIBIANA FABRE; MACALLINI, GUSTAVO; ONETO, ADRIANA; GONZALEZ, DIEGO; HIRSCHLER, VALERIA; ARANDA, CLAUDIO; BERG, GABRIELA
Revista:
Endocrine Connections
Editorial:
Sociedad Europea de Endocrinologia
Referencias:
Año: 2012 vol. 1 p. 58 - 61
ISSN:
2049-3614
Resumen:
Background: Saliva is a useful sample as a source of
hormones for the diagnosis of different
diseases, particularly in pediatric patients
and aged individuals, because saliva offers a
noninvasive and stress-free alternative to
serum collection. The aim of this study was to
validate a salivary insulin method and to
check its clinical application in pediatric patients.
Methods: Saliva samples were collected from
130 boys and 147 girls aged 614 years. Salivary
and serum insulin levels were measured with
the chemiluminescent automated method
Access (Beckman Coulter, Brea, CA, USA).
Serum blood glucose levels were measured with
the glucose oxidase method in an
autoanalyzer.
Results: The precision profile of the method
was determined for six aliquots of different
concentrations from pools of saliva, and the
coefficients of variation (CV) were 2.4% for
1 mUI/ml, 4% for 0.5, 8.9% for 0.25, 19% for 0.12, 28% for 0.06, and 38%
for 0.03 mUI/ml,
being the functional sensibility
(concentration corresponding to a 20% CV) 0.12 mUI/ml.
Insulin recovery was 100.13%. Salivary
insulin levels diminished 29.8% in samples stored
during 7 days at 28 8C. Differences in insulin values were not observed
when samples were
stored at K20 8C during 7 days. The
methods used to measure salivary and serum insulin
correlated significantly (rZ0.92, P!0.001). However, at
levels of serum insulin O20 mUI/ml,
this correlation declined (rZ0.57, PZ0.083).
Conclusion: The proposed method for salivary
insulin measurement showed convenient
analytical
characteristics.