QUALITY ASSURANCE OF SAMPLES AND PROCESSES IN THE SPANISH RENAL RESEARCH NETWORK (REDinREN) BIOBANK

MARÍA A. CORTÉS; LAURA CALLEROS; ANDREA GARCIA JEREZ; ALICIA LUENGO; ANA OROZCO-AGUDO; JOSÉ MANUEL VALDIVIELSO; DIEGO RODRIGUEZ-PUYOL; MANUEL RODRIGUEZ-PUYOL

BIOPRESERVATION AND BIOBANKING

MARY ANN LIEBERT INC

Lugar: New York; Año: 2016 vol. 14 p. 499 - 510

1947-5543

Biobanks are useful platforms to build bridges between basic, translational and clinical research and care practice. They are created as repositories of high-quality human biological samples ideal for evaluating the genome, transcriptome and proteome. This is the case of the Spanish Renal Research Network Biobank, which has more than 76,500 well-preserved frozen samples of a wide variety of kidney diseases, collected from over 70 nephrology services throughout theSpanish territory, obtained from specimens collected to 5,450 patients. Objectives: to determine and to report the results of the quality control of samples and processes conducted in our biobank, implemented on a routine basis in accordance with the requirements of ISO 9001:2008 international standard. Design & Methods and Results: This study showed that Peripheral Blood Mononuclear Cells isolation by Ficoll yielded excellent and reproducible results and their cryopreserved viability was greater than 90%. With regard to nucleic acids, acceptable A260/A280 ratios were obtained for the vast majority of the DNA (n=2328) and RNA (n=78) analyzed. In addition, DNA integrity was demonstrated by agarose gels and by PCR amplification of large fragments of β-globin gene, with DNA of acceptable quality with at least three bands of β-globin amplified obtained (n=26/30). RNA integrity was studied by RNA integrity number (RIN) determination, with very goods RIN numbers ≥ 7 obtained (n=87/96).The usability of both nucleic acids for gene amplification was confirmed by cuantitative PCR and RT-PCR of β-actin and GAPDH genes. Storage time did not affect the quality of the samples analyzed in any of the cases. The processes of DNA extraction also yielded reproducible results. Conclusions: these results clearly indicate that the samples stored in our biobank meet the required quality standards to be used for biomedical research, and ensure their long-term preservation.