LAGORIO MarÍa Gabriela
congresos y reuniones científicas
Non-destructive methods for assessment of senescence photochemistry: from leaf to stand level
GABRIELA B. CORDON; MARÍA GABRIELA LAGORIO; JOSÉ PARUELO
Congreso; 16th International Congress on Photobiology; 2014
International Union of Photobiology
Senescence is a key process in plants controlled by onthogeny but also by environmental factors. A reduction in chlorophyll concentration and in photosynthetic activity characerize senescence. The consequence of senescence of different functional aspects is critical to understant the influence of diverse stress factors. In this study senescence was evaluated in two grasses (Setaria italica a C4 species and Avena sativa C3 species) by non-destructive methods: reflectance spectroscopy and chlorophyll fluorescence to evaluate changes in pigments and on radiation use efficiency (RUE) and interception. Measures were performed at two level: individual leaves and the whole canopy. Both species were seeded and grown in pots. Radiance and reflectance spectra were recorded with a portable spectroradiometer FieldSpec Pro FR ASD equipped with leaf-clip accessories. From them, two spectral indices: PRI (a measure of RUE) and NDVI (a measure of photosynthetic active radiation absorption) were calculated. Fraunhofer Line Discrimination (FLD) method was used to extract the sun induced fluorescence (SIF) from the small canopy in the telluric O2-A absorption line (760 nm). Photochemical parameters (Fv/Fm, fPSII, qP, qNP, QNP) were obtained with a modulated fluorometer Hansatech. Measurements were performed on non-detached leaves. Pigments contents were also determined . Parallel to the decrease in total chlorophyll content (nmol/cm2) an increase in carotenoid/total chlorophyll ratio (Car/Chlor) was observed. The leaf PRI values decreased over time for both studied species. Canopy level values were more sensitive. NDVI values were less responsive. Canopy SIF (760 nm) decreased in time. We found that canopy level PRI correlated linearly and directly with SIF, Fv/Fm and fPSII for Avena sativa, Setaria italica and both species combined; while inversely related with qNP, QNP and Car/Chlor.