Protein subnetworks involving sHSPs during acclimatization process in living organisms.

ARCE D; PONCE, SERGIO D; TAPIA, ELIZABETH; PRATTA, GUILLERMO

Praga

Congreso; World Congress on Medical Physics & Biomedical Engineering; 2018

IUPESM

Small heat shock proteins (sHSPs) are low molecular weight (12?40 kDa) chaperones that have been associated with stress tolerance by preventing irreversible aggregation of misfolded proteins. Abiotic stress and developmental changes trigger sHSP gene expression and protein synthesis in all living organisms, suggesting the existence of a complex chaperone-network to maintain cellular homeostasis after heat stress (HS) exposure. Acclimatization process is characterized by the exposition to mild HS (35-38 °C) before severe HS (41-45 °C) exposition and is related to chronic adaptation to abiotic stress conditions. However, acclimatization might be involved in the adaptation of plants and animals to global warming. Identifying key regulatory and master regulators during this process, might contribute to agricultural and medical areas for better breeding programs coping with global warming. In order to describe which are the possible strategies developed by different organisms to face HS, we made an exhaustive bibliography curation process to identify experiments of HS, in the presence or absence of an acclimatization process. After assigning the corresponding Uniprot ID of co-regulated genes up-regulated in non-acclimated and acclimated HS organisms, predicted protein-protein interaction analysis (PPIs)were performed using STRING database. We identified similar gene chaperone network members,mainly represented by HSPs: HSP100, HSP90, HSP70 and sHSPs. Notably, a conservative subset of sHSPs are shared between co-expressed genes during HS in different model and nonmodel organisms (human, drosophila, wheat, Arabidopsis, rice, tomato and potato). Particularly, at least one of each mitochondrial, chloroplastic and cytosolic class I sHSPs, were present in all data-sets analyzed. Despite small variations in their order of appearance, HSP90, HSP70 and HSP100 proteins were identified and constitutes the first barrier or fence in response to HS conditions in different organisms. Our findings suggests that a conservative sub-network could be involved specifically during acclimatization in living organisms.