In Silico Analysis of Small Heat Shock Protein Gene Family by RNA-Seq during Tomato Fruit Ripening

ARCE, DÉBORA P; KRSTICEVIC, FLAVIA J; BERTOLACCINI, MARCO R; EZPELETA, JOAQUÍN; VALLE, ESTELA MARTA; PONCE, SERGIO DAMIÁN; TAPIA, ELIZABETH

Paris

Conferencia; 15FR01ICBCBBE_XIII International Conference on Bioinformatics, Computational Biology and Biomedical Engineering; 2015

WASET

Small Heat Shock Proteins (sHSPs) are low molecular weight chaperones that play an important role during stress response and development in all living organisms. Fruit maturation and oxidative stress can induce sHSP synthesis both in Arabidopsis and tomato plants. RNA-Seq technology is becoming widely used in various transcriptomics studies; however, analyzing and interpreting the RNA-Seq data face serious challenges. In the present work, we de novo assembled the Solanumlycopersicum transcriptome for three different maturation stages (mature green, breaker and red ripe). Differential gene expression analysis was carried out during tomato fruit development. We identified 4 sHSPs differentially expressed that might be involved in breaker and red ripe fruit maturation. Interestingly, these sHSPs have different subcellular localization and suggest a complex regulation of the fruit maturation network process