Isolation and characterization of a potato cDNA corresponding to a 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene differentially activated by stress.

ZANETTI, ME; TERRILE, MARÍA CECILIA; ARCE, DÉBORA; GODOY, ANDREA VERÓNICA; SAN SEGUNDO, BLANCA; CASALONGUÉ, CLAUDIA ANAHÍ

JOURNAL OF EXPERIMENTAL BOTANY

OXFORD UNIV PRESS

Lugar: Oxford; Año: 2002 vol. 53 p. 2455 - 2457

0022-0957

Aminocyclopropane-1-carboxylate (ACC) oxidase enzyme catalyses the final step in ethylene biosynthesis, converting 1-aminocyclopropane-1-carboxylic acid to ethylene. A cDNA clone encoding an ACC oxidase, ST-ACO3, was isolated from potato (Solanum tuberosum L.) by differential screening of a Fusarium eumartii infected-tuber cDNA library. The deduced amino acid sequence exhibited similarity to other ACC oxidase proteins from several plants species. Northern blot analysis revealed that the ST-ACO3 mRNA level increased in potato tubers upon inoculation with F. eumartii, as well as after treatment with salicylic acid and indole-3-acetic acid, suggesting a cross-talk between different signalling pathways involved in the defence response of potato tubers against F. eumartii attack.