INVESTIGADORES
GUTNISKY Cynthia
artículos
Título:
Morphological, biochemical and functional studies to evaluate bovine oocyte vitrification Article Type: Original Research Article
Autor/es:
CYNTHIA GUTNISKY; MORADO SERGIO; GADZE TOMAS; DONATO ANTONELLA; ALVAREZ GABRIEL; DALVIT GABRIEL; CETICA PABLO
Revista:
THERIOGENOLOGY
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Lugar: Amsterdam; Año: 2020
ISSN:
0093-691X
Resumen:
The aim of the present study was to evaluate the effect ofvitrification on morphological, biochemical and functional parameters ofmatured bovine oocytes at different recovery times. To this end, maturedbovine oocytes were vitrified using the Cryotech® kit (a minimum-volumesystem) and then incubated in maturation medium for different postwarmingdurations (0 h, 3 h or 21 h). Morphology, viability andbiochemical parameters were assessed at each time point mentioned aboveand the recovery of the metaphase plate was analyzed at 2 h, 3 h and 4 hpost-warming. The vitrification-warming process did not affect theviability or morphology of oocytes at any time point. However, therecovery of the metaphase plate occurred mostly between 3 and 4 h ratherthan at 2 h after warming (P
