Modulation of glycolysis and the pentose phosphate pathway influences porcine oocyte in vitro maturation

ALVAREZ GABRIEL; FERRETTI EUGENIA; GUTNISKY CYNTHIA; DALVIT GABRIEL; CETICA PABLO

REPRODUCTION IN DOMESTIC ANIMALS (1990)

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2012 vol. 48 p. 545 - 553

0936-6768

Glycolytic and pentose phosphate pathway (PPP) activities were modulated in porcine 29 cumulus-oocyte complexes (COCs) during in vitro maturation (IVM) by the addition of 30 inhibitors or stimulators of key enzymes of the pathways in order to elucidate their 31 relative participation in oocyte maturation. The activities of glycolysis and PPP were 32 evaluated by lactate production per COC and by the Brilliant Cresyl Blue (BCB) test, 33 respectively. Glucose uptake per COC and the oocyte maturation rate were also 34 evaluated. Lactate production, glucose uptake and the percentage of oocytes reaching 35 metaphase II decreased in a dose-dependent manner in the presence of the 36 pharmacological (NaF) or the physiological (ATP) inhibitors of glycolysis (P