Variations in metabolic parameters of in vitro matured porcine oocytes after vitrification-warming

MORADO SERGIO; APARICIO AILEN; PINCHETTI DANIELA; ARRAZTOA CLAUDIA CECILIA; ALVAREZ GABRIEL; GUTNISKY CYNTHIA; NEILD DEBORA; DALVIT GABRIEL; CETICA PABLO

Open Veterinary Journal

Faculty of Veterinary Medicine University of Tripoli and Eldaghayes Publ

Año: 2023

2218-6050

Background: As the porcine oocyte is the most sensitive to low temperature damage, it has been difficult to cryopreserve compared to those from other domestic animals. However, at present, vitrification is used as a method for the cryopreservation of both oocytes and embryos in this species.Aim: Our aim was to analyze alterations in metabolic parameters in vitrified-warmed in vitro matured porcine oocytes at different post-warming recuperation times. Additionally, metaphase II plate recovery time analysis, in vitro fertilization and intracytoplasmic sperm injection were carried out to evaluate oocyte recovery capacity. Methods: Oocytes were vitrified-warmed and then incubated for 0, 3 or 21h post-warming to assess biochemical parameters. Results: Oocyte viability and morphology were not affected by vitrification-warming. Cytosolic oxidative status, active mitochondria and reactive oxygen species levels presented changes at the different time points in control and vitrified-warmed oocytes (P