? ?EFFECT OF METRONIDAZOLE ON IN VITRO ISOLATES OF Tritrichomonas foetus?

RIVERO, MB; LUQUE, ME; ABDALA, ME; CARRANZA, PG; RIVERO, FD

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

LXII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA

"Bovine Trichomonosis" (BT), caused by infection with the flagellated parasite Tritrichomonas foetus, is a sexually transmitted disease limited to the urogenital tract of cattle. In Argentina, BT is endemic and a relevant health problem as it causes reproductive inefficiency in cattle, which is accompanied by large economic losses. For decades, Metronidazole has been the drug of choice in the treatment of BT. However, more recently both aerobic and anaerobic resistance have been reported, in vivo and in vitro, respectively. Presently, little is known as to Metronidazole values for minimal lethal (MLC) and minimum inhibitory (MIC) concentrations against these parasites. Thus, the goal of the present study was to explore the susceptibility of six different isolates of T. foetus under aerobic (AC) and anaerobic (ANC) conditions. Isolates were obtained from three Argentine provinces, Buenos Aires (1), Salta (4) and Santa Fe (1). The 24-h exposure times of isolates to the drug rendered MLC values in the range of 2.5 - 5 μg/mL and ≥5 μg/mL for ANC and AC conditions, respectively, while the values after 48-h were 1.25 ? 2.5 μg/mL and 2.5 and 5 μg/mL, for ANC and AC conditions, respectively. MIC values after 24 h were between 1.25 - 2.5 μg/mL and 2.5 and >5 μg/mL for ANC and AC, respectively, while the 48 h values were between 0.625 and 1.25 μg/mL and 1.25 and 2.5 μg/mL for ANC and AC, respectively.The present data provide updated information on T. foetus susceptibility to Metronidazole, which reveal both MLC and MIC higher values than those previously reported in available bibliographic records.