Induction of Both Local Immune Response in Mice and Protection in a Rabbit Model by Intranasal Immunization with Modified Vaccinia Ankara Virus Expressing a Secreted Form of Bovine Herpesvirus 1 Glycoprotein D

MARIA PAULA DEL MEDICO ZAJAC, FLAVIA ADRIANA ZANETTI, MARÍA SOLEDAD ESUSY, CARLOS RODOLFO FEDERICO,; OSVALDO ZABAL; ALEJANDRO RAFAEL VALERA; GABRIELA CALAMANTE

VIRAL IMMUNOLOGY

MARY ANN LIEBERT INC

Lugar: New York; Año: 2017 vol. 30 p. 70 - 76

0882-8245

In this study, we evaluated the immunogenicity and efficacy of mucosal delivery of a recombinant modified vaccinia Ankara virus (MVA) expressing the secreted version of bovine herpesvirus type 1 (BoHV-1) glycoprotein D (MVA-gDs) without addition of adjuvant in two animal models. First, we demonstrated the capabilityof MVA-gDs of inducing both local and systemic anti-gD humoral immune response after intranasal immunization of mice. Then, we confirmed that two doses of MVA-gDs administered intranasally to rabbits induced systemic anti-gD antibodies and conferred protection against BoHV-1 challenge. Our results show the potentialof using MVA as a vector for the rational design of veterinary vaccines capable of inducing specific and protective immune responses both at local and systemic level.