Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates

ENCINAS, M.; MARFIL, J.; GARBACCIO, S.; BARANDIARAN, S.; HUERTAS, P.; MORSELLA, C.; MACÍAS, A.; MAGNANO, G.; ZAPATA, L.; BIGI, F.; CATALDI, A.; PAOLICCHI, F.; ZUMÁRRAGA, M.; EIRIN, M.E.

Tuberculosis (Edinb)

CHURCHILL LIVINGSTONE

Año: 2018

1472-9792

ESAT-6, CFP-10 and EspC are virulence factors that have been extensively assayed for bovine andhuman tuberculosis diagnosis due their potent T-cell inducing activities. While polymorphisms ofESAT-6 and CFP-10 were analyzed, with the description of CFP-10 variants in M. tuberculosis, thisfact has not been explored in M. bovis field isolates. The coding sequences of esxA (ESAT-6), esxB(CFP-10) and mb3645c (EspC) from 58 M. bovis strains exhibiting genomic variability (spoligotyping)were analyzed. Two genes ?esxA and esxB ? remained invariant while mb3645c exhibited onesynonymous polymorphism (G to A mutation, position 66bp) in one isolate, compared to M. bovisAF2122/97 reference strain. All isolates exhibited a synonymous nucleotide polymorphismsimultaneously (G to A mutation, position 255bp), compared to M. tuberculosis H37Rv referencestrain. This study confirms the high conservation for ESAT-6, CFP-10 and EspC in local M. bovis fieldisolates and reinforce the use of these three antigens in the diagnosis of bovine tuberculosis. Further studies should be performed to globally confirm these findings.