Differential transcriptome profiles of attenuated and hypervirulent strains of Mycobacterium bovis

BLANCO, FEDERICO CARLOS; NÚÑEZ GARCÍA, JAVIER; GARCÍA-PELAYO, CARMEN; SORIA, MARCELO; BIANCO, MARÍA VERÓNICA; ZUMÁRRAGA, MARTÍN JOSÉ; GOLBY, PAUL; CATALDI, ANGEL; GORDON, STEPHEN V.; BIGI, FABIANA

MICROBES AND INFECTION

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2009 vol. 11 p. 956 - 963

1286-4579

The identification of factors involved in the interaction of Mycobacterium bovis with the hosts will lead to new strategies to control bovine tuberculosis. In this study we compared the transcriptional profile of an attenuated M. bovis strain and a virulent M. bovis strain as a means to elucidate the molecular basis for their differential phenotype. Microarray and RT-qPCR results demonstrated that the expression of mce4D, Mb2607/Mb2608 and Mb3706c were up-regulated in the virulent strain whereas alkB, Mb3277c and Mb1077c were expressed at higher levels in the attenuated strain. These differential expression profiles were confirmed for Mb2607/Mb2608, mce4D, Mb1077c, alkB and Mb3277c during the replication of bacteria inside macrophages.