Mycobacterium bovis: Polymerase Chain Reaction, identification in bovine lymphonode

ZANINI, MARCOS SANTOS; MOREIRA, E. C; LOPES, M. T. P.; OLIVEIRA, R. S.; LEAO, SYLVIA CARDOSO; FIORAVANTI, R.L.; ROXO, ELIANA; ZUMÁRRAGA, MARTÍN JOSÉ; ROMANO, MARÍA ISABEL; CATALDI, ANGEL; SALAS, CARLOS E.

MEMóRIAS DO INSTITUTO OSWALDO CRUZ.

FUNDACO OSWALDO CRUZ

Lugar: Rio de Janeiro; Año: 2001 vol. 96 p. 809 - 813

0074-0276

Diagnosis of the Mycobacterium tuberculosis complex by direct PCR of mediastinal lymphnode DNA and microbiological tests were compared in cattle suspicious of bearing tuberculous-like lesions detected during slaughter. The PCR procedure applied on DNA samples (n=54) obtained by adding alpha -casein into the thiocyanate extraction mix was positive in 70% of the samples. PCR confirmed the identification of 23 samples (100%) that grew in culture, 9 samples (60%) that failed to grow in culture, plus 6 (37.5%) samples that resulted in growth of bacterial contaminants. Genotyping by IS6110-RFLP and DR-spoligotyping analysis of seven samples revealed the presence of several polimorphisms. Seven of the isolates contained multiple copies of IS6110, thus defining the existence of five singular genotypes.